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Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis Anais da ABC (AABC)
DREYFUSS,JULIANA L.; REGATIERI,CAIO V.; COELHO,BRUNO; BARBOSA,JOSÉ B.; FREITAS,DENISE DE; NADER,HELENA B.; MARTINS,JOÃO R..
The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conjunctivitis; Glycosaminoglycans; Hyaluronic acid; Tear film.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455
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Effectiveness of Origanum vulgare L. and Origanum majorana L. essential oils in inhibiting the growth of bacterial strains isolated from the patients with conjunctivitis BABT
Oliveira,Jana Luíza Toscano Mendes de; Diniz,Margareth de Fátima Melo; Lima,Edeltrudes de Oliveira; Souza,Evandro Leite de; Trajano,Vinícius Nogueira; Santos,Bernadete Helena Cavalcante.
This study aimed to evaluate the antibacterial activity of Origanum vulgare L. and O. majorana L. essential oils on Staphylococcus aureus, S. coagulase negative, Enterobacter spp., Proteus spp., Acinetobacter spp., Klebsiella spp. isolated from the patients with conjunctivitis. The results showed a prominent inhibitory effect of both the essential oils on all the bacterial strains, noted by the large bacterial growth inhibition zones (15-32mm). The Minimum Inhibitory Concentrations (MIC) values were between 5-20µL/mL and 2.5-10 µL/mL for O. vulgare and O. majorana essential oil, respectively. The MIC were able to cause significant (P<0.05) inhibitory effect on the cell viability of Klebsiella spp., Proteus spp. and S. aureus providing a total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Origanum vulgare L.; Origanum marjorana L.; Essential oil; Antibacterial activity; Conjunctivitis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100006
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Feline herpesvirus 1 viral load related to environmental factors in sheltered cats Ciência Rural
Baumworcel,Natasha; Pereira,Joylson de Jesus; Soares,Ana Maria Barros; Souza,Guilherme Nunes; Almosny,Nadia Regina Pereira; Castro,Tatiana Xavier de.
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection....
Tipo: Info:eu-repo/semantics/article Palavras-chave: FHV-1; Conjunctivitis; Shelter medicine; QPCR; Animal shelter.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454
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Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil BJM
Pereira,Joylson de Jesus; Baumworcel,Natasha; Fioretti,Júlia Monassa; Domingues,Cinthya Fonseca; Moraes,Laís Fernandes de; Marinho,Robson dos Santos Souza; Vieira,Maria Clara Rodrigues; Pinto,Ana Maria Viana; de Castro,Tatiana Xavier.
ABSTRACT The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conjunctivitis; FCV genogroup; Household; Molecular characterization; Shelter.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400777
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Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study BJID
Akçay,Emine; Çarhan,Ahmet; Hondur,Gözde; Tufan,Zeliha Koçak; Duru,Necati; Kılıç,Selçuk; Ensari,Ezgi Naz; Uğurlu,Nagihan; Çağıl,Nurullah.
Abstract Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenovirus; Conjunctivitis; Viral; PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400391
Registros recuperados: 5
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